Journal: Aging and Disease

Article Title: Stroke Exacerbates Respiratory Disorder and Cognition Impairment in Mice with Cerebral Amyloid Angiopathy

doi: 10.14336/AD.2025.0474

Figure Lengend Snippet: Stroke increases astrocytic gliosis and induced astrocytic polarization in RTN. (A) RTN chemo-sensitive phox2B-positive neurons (area circumscribed by red solid line) are located ventromedial to the VII, medial to the pyramidal tract. (B) Histological representation of the RTN location within the brainstem, illustrating the specific area analyzed in both WT, CAA, and stroke mice, in relation to the 7th Facial Nucleus (7 N), Scale bar = 300μm/50 μm (zoom). (C-G) Images show the GFAP expression in each group(F (2,18) =19.043, P ＜0.001), and dual-IF staining for GFAP with C3 (F (2,18) =19.851, P ＜0.001 ), GFAP with S100A10 (F (2,18) =21.306, P ＜0.001) in RTN from WT, CAA, and stroke mice at day 42 post-ischemia (n= 5 for WT group, n=7 for CAA-Sham group and n=9 for CAA-pd-MCAO group). Scale bar = 50 µm. * P <0.05; ** P <0.01; *** P <0.001. ns: non-significance. The data are shown as the mean ± SEM. After performing the Shapiro-Wilk normality test to examine the normal distribution, One-way analysis of variance (ANOVA) was employed to analyze the data pertaining to multiple groups, subsequently, multiple comparisons were conducted using the uncorrected Fisher's LSD test.

Article Snippet: Sections were then incubated overnight at 4°C with primary antibodies against Aβ (1:300, Abcam, Cat# ab201060), Phox2B (1:20, Bio-Techne, Cat# AF4940), GFAP (1:200, Novus, Cat# NB100-53809), C3 (1:200, Abcam, Cat# ab97462) S100A10 (1:200, Invitrogen, Cat# PA5-95505), LYVE1(1:200, CST, Cat# E3L3V) and in the blocking solution.

Techniques: Expressing, Staining